CJC 1295 NO DAC 5mg

Description CJC-1295 No DAC (Modified GRF 1-29) – 5mg – A Growth Hormone-Releasing Peptide for Endocrine and Metabolic Research Category: Peptide Research Compounds / GHRH Analogs / Endocrine & Metabolic Studies Disclaimer: ATTENTION: ELITEBIOGENIX.COM PRODUCTS ARE INTENDED FOR LABORATORY RESEARCH AND IN-VITRO STUDIES ONLY. All information presented on this site is for educational and research purposes related to scientific investigation. These products are strictly not for human or veterinary use, consumption, or diagnostic applications. They are not pharmaceuticals, food additives, or dietary supplements. By purchasing from EliteBiogenix.com, you acknowledge and affirm that you are a qualified researcher operating in a controlled laboratory environment. You must adhere to all applicable local, state, and federal laws and regulations regarding the handling and use of research chemicals. Handle all materials with appropriate personal protective equipment (PPE) and under laboratory safety protocols. A Precise Tool for Pulsatile GH Research: A Guide to CJC-1295 No DAC Welcome, esteemed researchers, to EliteBiogenix’s detailed examination of a refined growth hormone-releasing peptide: CJC-1295 No DAC (also known as Modified GRF 1-29 or Tetrasubstituted GRF). This product contains 5mg of high-purity, lyophilized peptide, representing a precise tool for investigating the dynamics of the hypothalamic-pituitary axis. CJC-1295 No DAC is a synthetic analog of Growth Hormone-Releasing Hormone (GHRH), modified for enhanced stability and receptor binding, while specifically lacking the Drug Affinity Complex (DAC) moiety that confers extended duration of action. This deliberate design allows researchers to study pulsatile growth hormone (GH) release patterns without the sustained elevation associated with the DAC version. This product description serves as a foundational research primer to guide your experimental design in endocrinology, metabolism, and cellular signaling. Section 1: The Scientific Basis of CJC-1295 No DAC’s Mechanism of Action The research value of CJC-1295 No DAC lies in its targeted interaction with the growth hormone-releasing hormone receptor (GHRH-R) and its ability to stimulate natural, pulsatile GH secretion patterns. Targeted GHRH Receptor Agonism: CJC-1295 No DAC is a potent, synthetic agonist of the GHRH receptor, which is primarily expressed on somatotroph cells in the anterior pituitary gland. Upon binding to this G-protein-coupled receptor, it initiates a signaling cascade involving cyclic AMP (cAMP) and protein kinase A (PKA), leading to the synthesis and secretion of growth hormone (GH) from storage vesicles. Enhanced Stability Through Structural Modification: The peptide is a tetra-substituted analog of the endogenous GHRH (1-29) sequence. It incorporates modifications (specifically, substituting the first two amino acids with D-Ala and modifying positions 8, 15, and 27) that confer resistance to enzymatic degradation by dipeptidyl peptidase-4 (DPP-4). This results in a significantly extended half-life in experimental models compared to native GHRH (1-29), allowing for more sustained receptor activation in research settings. Absence of DAC for Natural Pulsatility: Unlike its counterpart CJC-1295 with DAC, the “No DAC” version lacks the Drug Affinity Complex. The DAC moiety is designed to covalently bind to serum albumin, creating a depot effect and prolonging the molecule’s circulation time to over a week. By omitting this, CJC-1295 No DAC maintains a shorter, more physiological-like half-life, enabling researchers to study the effects of discrete, pulsatile GH stimulation—a critical feature of normal GH physiology—rather than continuous GH elevation. Section 2: Key Research Applications for CJC-1295 No DAC Our 5mg vial of lyophilized CJC-1295 No DAC is designed for controlled in-vitro and ex-vivo research applications. The following sections outline primary research domains. 2.1 Investigating Pituitary Somatotroph Function The direct effect of GHRH analogs on pituitary cells is a foundational area of research. Research Application: Researchers can use our CJC-1295 No DAC in primary pituitary cell cultures or immortalized somatotroph cell lines (e.g., GH3 cells). Key endpoints include: GH Secretion Measurement: Quantifying GH release into culture media via ELISA or radioimmunoassay (RIA) following peptide treatment. Intracellular Signaling Analysis: Using Western blot or phospho-flow cytometry to study activation of the cAMP/PKA pathway and downstream transcription factors. Gene Expression: Measuring changes in GH gene transcription using qPCR. Estimated Compound Usage for In-Vitro Replication: Effective concentrations in pituitary cell cultures typically range from 1 nM to 100 nM. For a 1 mL culture well treated at 10 nM, this requires approximately 10 ng of CJC-1295 No DAC. Our 5mg vial allows for approximately 500,000 such individual wells at this concentration, enabling extensive dose-response and time-course studies. 2.2 Comparative Studies: No DAC vs. DAC and Other GHRH Analogs A critical research application is the direct comparison of CJC-1295 No DAC with its DAC-containing counterpart or with endogenous GHRH. Research Application: Side-by-side experiments in the same cell culture or explant system can definitively characterize the differences in GH release kinetics, receptor desensitization rates, and downstream signaling profiles. Experimental Design: Treat pituitary cells with equimolar concentrations of CJC-1295 No DAC, CJC-1295 with DAC, and native GHRH. Measure GH secretion at multiple time points (e.g., 1, 6, 12, 24 hours) to compare the duration and amplitude of the secretory response. 2.3 Studies on Pulsatile GH Release and Metabolic Outcomes The ability of CJC-1295 No DAC to mimic natural pulsatile GH release makes it a valuable tool for studying the metabolic effects of GH pulses versus continuous GH exposure. Research Application: In more complex in-vitro systems, such as perfused pituitary columns or co-cultures of hypothalamic neurons and pituitary cells, researchers can apply CJC-1295 No DAC in pulses to model the natural rhythm of GHRH secretion. Downstream effects can be studied on: Hepatocyte (Liver Cell) Cultures: Measuring IGF-1 production and metabolic gene expression in hepatocytes exposed to GH-rich media collected from stimulated pituitary cultures. Adipocyte Cultures: Studying the lipolytic effects of pulsatile vs. continuous GH exposure. Section 3: Synergistic Research: CJC-1295 No DAC and Other Compounds With Ghrelin Analogs (e.g., GHRP-2, GHRP-6, Ipamorelin): GHRP compounds act on the ghrelin receptor (GHS-R) and synergistically enhance GH release when combined with GHRH analogs. Research can investigate the synergistic potential of combining CJC-1295 No DAC with various GHRPs on GH secretion from pituitary cultures. With Somatostatin Analogs: Research can explore the opposing effects of GHRH agonists and somatostatin analogs on GH release, modeling the dynamic balance of the hypothalamic-pituitary axis. Section 4: EliteBiogenix Quality and Specifications Product Specification: Compound Name: CJC-1295 No DAC (Modified GRF 1-29, Tetrasubstituted GRF) Sequence: Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH₂ Molecular Weight: ~3367.9 Da Purity: ≥98% (HPLC Verified) Form: White lyophilized powder Storage: Store at -20°C or below. Desiccate. Protect from light. Solubility: Soluble in sterile water, 0.9% saline, or bacteriostatic water. For cell culture, filter sterilize through a 0.22 micron low-protein-binding filter after reconstitution. Why Choose EliteBiogenix for Your CJC-1295 No DAC Research? Critical Purity: Our ≥98% purity ensures that research results are attributable to the peptide of interest, not impurities. Validated for In-Vitro Use: Each batch is quality-controlled for use in sensitive cell culture systems. Research-Focused Supply: The 5mg size is ideal for conducting a full series of in-vitro experiments, from dose-finding to mechanistic signaling studies. Section 5: Conclusion This 5mg vial of research-grade CJC-1295 No DAC from EliteBiogenix provides a precise tool for investigating the dynamics of the growth hormone axis. Its targeted action on pituitary somatotrophs and its design for pulsatile stimulation make it an invaluable compound for endocrinology, metabolic, and cellular signaling research. Order your vial of CJC-1295 No DAC (5mg) today to advance your research into growth hormone regulation and metabolic physiology. Remember: EliteBiogenix.com products are for laboratory research use only. Not for human or veterinary consumptiin.